The study of RNA has been dramatically improved by the introduction of Next Generation Sequencing platforms allowing massive and cheap sequencing of selected RNA fractions, also providing information on strand orientation (RNA-Seq). The complexity of transcriptomes and of their regulative pathways make RNA-Seq one of most complex field of NGS applications, addressing several aspects of the expression process (e.g. identification and quantification of expressed genes and transcripts, alternative splicing and polyadenylation, fusion genes and trans-splicing, post-transcriptional events, etc.). In order to provide researchers with an effective and friendly resource for analyzing RNA-Seq data, we present here RAP (RNA-Seq Analysis Pipeline), a cloud computing web application implementing a complete but modular analysis workflow. This pipeline integrates both state-of-the-art bioinformatics tools for RNA-Seq analysis and in-house developed scripts to offer to the user a comprehensive strategy for data analysis. RAP is able to perform quality checks (adopting FastQC and NGS QC Toolkit), identify and quantify expressed genes and transcripts (with Tophat, Cufflinks and HTSeq), detect alternative splicing events (using SpliceTrap) and chimeric transcripts (with ChimeraScan).
I have a SketchUp Pro 2017 license on my Windows 10 Home and a SketchUp Pro 2016 license on my Windows 8.1. I used to be able to download more than one file per license from 3D Warehouse at the same time. Now I can only download one at a time and I cannot download more than one SketchUp Pro 2017 license... any idea?
The Sumita Arora python class 12 book is an ideal study guide for students who are preparing for the competitive exams. It includes concepts that are designed to help students build a strong foundation for the competitive exams and prepare them to build their careers as professionals. You can also use the Sumita Arora python class 12 book to prepare for competitive exams like Olympiad, GATE, and IIT-JEE. 827ec27edc